IgM Heavy Chain
نویسندگان
چکیده
منابع مشابه
Lineage-restricted retention of a primitive immunoglobulin heavy chain isotype within the Dipnoi reveals an evolutionary paradox.
The lineage leading to lungfishes is one of the few major jawed vertebrate groups in which Ig heavy chain isotype structure has not been investigated at the genetic level. In this study, we have characterized three different Ig heavy chain isotypes of the African lungfish, Protopterus aethiopicus, including an IgM-type heavy chain and short and long forms of non-IgM heavy chains. Northern blot ...
متن کاملEffects of a long-term spaceflight on immunoglobulin heavy chains of the urodele amphibian Pleurodeles waltl.
A variety of immune parameters are modified during and after a spaceflight. The effects of spaceflights on cellular immunity are well documented; however, little is known about the effects of these flights on humoral immunity. During the Genesis space experiment, two adult Pleurodeles waltl (urodele amphibian) stayed 5 mo onboard Mir and were subjected to oral immunization. Animals were killed ...
متن کاملTyrosine sulfation is a trans-Golgi-specific protein modification
The trans-Golgi has been recognized as having a key role in terminal glycosylation and sorting of proteins. Here we show that tyrosine sulfation, a frequent modification of secretory proteins, occurs specifically in the trans-Golgi. The heavy chain of immunoglobulin M (IgM) produced by hybridoma cells was found to contain tyrosine sulfate. This finding allowed the comparison of the state of sul...
متن کاملExpression of heavy chain‐only antibodies can support B‐cell development in light chain knockout chickens
Since the discovery of antibody-producing B cells in chickens six decades ago, chickens have been a model for B-cell development in gut-associated lymphoid tissue species. Here we describe targeting of the immunoglobulin light chain locus by homologous recombination in chicken primordial germ cells (PGCs) and generation of VJCL knockout chickens. In contrast to immunoglobulin heavy chain knocko...
متن کاملClass II-restricted presentation of an immunoglobulin heavy-chain-gene product by a gene-transfected B-cell line.
The presentation of an antigen endogenously processed by B lymphocytes was investigated. The expression plasmid vectors, harboring genomic rearranged V genes from two monoclonal B cells and genomic mu-constant region gene, were constructed. Two B-cell lines, the MOPC104E myeloma mu-heavy chain expressing AMB line and the control hybridoma mu-heavy chain expressing AHB line, were established by ...
متن کاملDetection of weak receptor–ligand interactions using IgM and J-chain-based fusion proteins
Materials and methods IgM-based fusion proteins were generated by molecular cloning of the IgM backbone DNA sequence into the BTN2A2-IgG or PD-L1-IgG-encoding SigpIg+ vectors upstream of the IgG1 Fc part of the fusion protein. The secretory tailpiece of the human IgM heavy chain was also encoded in the human IgM heavy chain DNA sequence inserted (Mitoma J. et al. Nat Immunol .2007.8: 409-418). ...
متن کامل